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European Journal of Gynaecological Oncology  2019, Vol. 40 Issue (4): 585-592    DOI: 10.12892/ejgo4484.2019
Original Research Previous articles | Next articles
The role of vitamin D up-regulated protein 1 in epithelial ovarian cancer
Dong-Hee Kim1, †, Seung-Do Choi2, †, Hae-Hyeog Lee3, *(), Tae-Hee Kim3, Yeon-Suk Kim4, Yesol Kim4
1 Department of Obstetrics and Gynecology, Green OBGY Clinic, Incheon
2 Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Cheonan
3 Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Republic of Korea
4 Department of Interdisciplinary Program in Biomedical Science, Soonchunhyang University, Asan, Republic of Korea
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Abstract  
Purpose: The objective of this study was to verify the relationship between the expression of VDP1 and Jun activation domain-binding protein 1 (Jab1) and cyclin-dependent kinase inhibitor 1B (CDKN1B: p27kip1) in ovarian cancer cell strains. Materials and Methods: The ovarian cancer cell lines SK-OV-3, OVCAR-3 were plated and cultivated for 24 hours in a 37°C carbon dioxide incubator. Thereafter, vitamin D was added at various concentrations, and the cells were cultivated for an additional 24 hours. Using the 3-(4.5- dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assay, cell growth was checked, and changes in the protein level were observed using Western blotting, immunofluorescence and immunocytochemistry. Results: The authors found that the viability of the ovarian cancer cells was decreased by vitamin D, dose dependently. The results of Western blotting, immunofluorescence, and immunocytochemistry revealed that p27kip1 and VDUP1 increased, while Jab1 decreased, dose dependently. Conclusion: These findings show the relationship between p27kip1, Jab1 and VDUP1 following vitamin D treatment in ovarian cancer, indicating a correlation with cancer cell proliferation.
Key words:  Epithelial ovarian neoplasms      Jab1      p27kip1      VDUP1 protein      Vitamin D     
Published:  10 August 2019     
*Corresponding Author(s):  HAE-HYEOG LEE     E-mail:  hhl22@schmc.ac.kr
About author:  † Contributed equally.

Cite this article: 

Dong-Hee Kim, Seung-Do Choi, Hae-Hyeog Lee, Tae-Hee Kim, Yeon-Suk Kim, Yesol Kim. The role of vitamin D up-regulated protein 1 in epithelial ovarian cancer. European Journal of Gynaecological Oncology, 2019, 40(4): 585-592.

URL: 

https://ejgo.imrpress.com/EN/10.12892/ejgo4484.2019     OR     https://ejgo.imrpress.com/EN/Y2019/V40/I4/585

[1] Arum Lee, Tae-Hee Kim, Hae-Hyeog Lee, Jun-Mo Kim, Yeon-Suk Kim. Expression of Jun activation domain-binding protein 1 and p27 Kip1 in endometrial polyps and uterine leiomyomas[J]. European Journal of Gynaecological Oncology, 2018, 39(4): 533-537.
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