Please wait a minute...
European Journal of Gynaecological Oncology  2020, Vol. 41 Issue (2): 214-220    DOI: 10.31083/j.ejgo.2020.02.5052
Original Research Previous articles | Next articles
Mifepristone regulates the multidrug resistance via miR-758/MEPE of Hela cell lines
Hong Yan1, †, Chunhua Zhang2, †, Tingting Liu3, Jianzhong Liu3, *()
1 Department of Pathology, The Second Hospital of Lanzhou University, Lanzhou 730030, China
2 Department of Gynaecology, Huaian Maternal and Child Health-Care Center, Jiangsu 223002, China
3 Department of Gynaecology and Obstetrics, Huaian Second People's Hospital The Affiliated Huaian of Xuzhou Medical University 223002, China
Download:  PDF(3130KB)  ( 275 ) Full text   ( 4 )
Export:  BibTeX | EndNote (RIS)      
Abstract  
Background: In cervical cancer, matrix extracellular phosphoglycoprotein (MEPE) plays an important role of multidrug resistance, which is associated with miR-758. Mifepristone has anti-drug resistance effects, but whether mifepristone regulates the expression of multidrug resistance proteins via the pathway mediated by miR-758/MEPE is still unclear. Hela cells were induced by mifepristone (named as Hela/MIF cells). Then, matrix extracellular phosphoglycoprotein siRNA, and miR-758 mimics were transfected into HeLa/MIF cells. The sensitivity, clone forming ability, migration, and level of apoptosis of the cells in each group were respectively measured by CCK-8 assays, clone forming assays, transwell assays, and flow cytometry analysis. The targeting of miR-758 to MEPE was verified by dual-luciferase assay. At last, the expression of MDR-1, MRP-1, and GST-π were detected by qPCR and western blot assays. Results: The induction by mifepristone not only decreased the sensitivity of Hela cells, but also up-regulated the expression of multidrug resistance proteins. On this basis, by increasing the expression of miR-758 or downregulating the expression of MEPE, the expression of multidrug resistance proteins decreased, while the sensitivity of Hela cells to mifepristone were improved and the level of Hela cells proliferation, colony forming, and invasion further decreased. Conclusions: The induction by mifepristone inhibited the proliferation of Hela cells, but the sensitivity of Hela cells increased. The mechanism may depend on the expression of multidrug resistance protein. This study shows that regulating the expression of miR-758 and MEPE can reduce the resistance of Hela cells to mifepristone, enhance the sensitivity, and further improve the inhibitory effect of mifepristone.
Key words:  Cervical cancer      Multidrug resistance      Mifepristone      MicroRNA      matrix extracellular phosphoglycoprotein     
Published:  15 April 2020     
*Corresponding Author(s):  JIANZHONG LIU     E-mail:  liujz1820@163.com
About author:  Contributed equally

Cite this article: 

Hong Yan, Chunhua Zhang, Tingting Liu, Jianzhong Liu. Mifepristone regulates the multidrug resistance via miR-758/MEPE of Hela cell lines. European Journal of Gynaecological Oncology, 2020, 41(2): 214-220.

URL: 

https://ejgo.imrpress.com/EN/10.31083/j.ejgo.2020.02.5052     OR     https://ejgo.imrpress.com/EN/Y2020/V41/I2/214

[1] Eda Kucuktulu, Ahmet Fatih Yurekli. Simultaneous Integrated Intensity Modulated Radiotherapy Boost for Gynecological Cancer when Brachytherapy is not an Option[J]. European Journal of Gynaecological Oncology, 2020, 41(5): 720-725.
[2] Wook Youn Kim, Shin Hee Seo, Seung-Hyuk Shim, Jin Hee Park, Hyung Kyu Park, Kyeong A So, Tae Jin Kim, Sun Joo Lee. Correlation of immunohistochemistry and silver in situ hybridization for the assessment of c-MET in uterine cervical cancer patients treated with radical hysterectomy[J]. European Journal of Gynaecological Oncology, 2020, 41(5): 745-752.
[3] John P. Micha, Howard D. Epstein, Michael C. Roossin, Randy Bohart, Mark A. Rettenmaier, Bram H. Goldstein. Neoadjuvant chemotherapy, radical hysterectomy and chemoradiation for stage iva cervical carcinoma[J]. European Journal of Gynaecological Oncology, 2020, 41(5): 797-801.
[4] Juan Xu, Qingqing Tan, Ting Li. USP22 promotes the expression of GLUT1 and HK2 to facilitate growth and glycolysis in cervical cancer cells[J]. European Journal of Gynaecological Oncology, 2020, 41(5): 790-796.
[5] Yan qin Zhang, Di Wu, Meng qi Deng, Xiang yu Chang, Jin wei Miao, Yu mei Wu. Individual management of cervical cancer during pregnancy[J]. European Journal of Gynaecological Oncology, 2020, 41(5): 833-837.
[6] Yi-fan Luo, Li-xiang Ren, Ming-yan Jiang, Yang Chu. A systematic review and meta-analysis on the effects of metformin on survival outcomes and risk in women with cervical cancer[J]. European Journal of Gynaecological Oncology, 2020, 41(4): 504-507.
[7] Liman Yang, Jing Jin, Juhong Liu. Correlation of MMP2-C1306T (rs243865) and MMP7-181A/G (rs11568818) with cervical cancer: a meta-analysis[J]. European Journal of Gynaecological Oncology, 2020, 41(4): 508-512.
[8] Panteleimon Mnimatidis, Abraham Pouliakis, George Valasoulis, George Michail, Aris Spathis, Christine Cottaridi, Niki Margari, Maria Kyrgiou, Maria Nasioutziki, Alexandros Daponte, Konstantinos Dinas, Evangelos Paraskevaidis, Ioannis Panayiotides, Dimitrios-Dionysios Koutsouris. Multicentric assessment of cervical HPV infection co-factors in a large cohort of Greek women[J]. European Journal of Gynaecological Oncology, 2020, 41(4): 545-555.
[9] Fengmei Wang , Huachun Luo , Huihua Cheng, Huijuan Huang , Zhichao Fu. Is 3D printing-gided three-dimensional brachytherapy suitable for cervical cancer: from one single research institute?[J]. European Journal of Gynaecological Oncology, 2020, 41(4): 591-597.
[10] Hiroshi Yoshida, Megumi Yamamoto, Hiroyuki Shigeta. Successful treatment of uterine cervical carcinoma with extensive vaginal lesions using laparoscopic surgery: A case report[J]. European Journal of Gynaecological Oncology, 2020, 41(4): 629-633.
[11] Hikari Unno, Seiji Mabuchi, Isao Arai, Tomoko Wakasa, Seiichi Yamamasu. Development of lymphangiosarcoma after pelvic radiation therapy for uterine cervical cancer: A case report and literature review[J]. European Journal of Gynaecological Oncology, 2020, 41(3): 326-331.
[12] L. Xu, H. Cai, N. Zhu, Bo Zheng. Interleukin-22 derived from cervical cancer-associated fibroblasts accelerates senescence of normal fibroblasts and promotes expression of tumorigenesis-related factors in HeLa cells[J]. European Journal of Gynaecological Oncology, 2020, 41(2): 192-199.
[13] K. Plagens–Rotman, M. Piskorz–Szymendera, B. PIĘTA. Lifestyle with particular emphasis on physical activity and genital carcinomas in women[J]. European Journal of Gynaecological Oncology, 2020, 41(2): 233-239.
[14] B. Bárány, R. Póka. Nutritional assessment among patients with cervical cancer and controls[J]. European Journal of Gynaecological Oncology, 2020, 41(1): 23-29.
[15] D. Kim, Y. Ki, W. Kim, D. Park, D. Suh, K. Kim, J. Lee, H. Jeon, J. Nam. Positive pelvic lymph node on [18F]-FDG PET is a prognostic factor in early-stage high-risk cervical cancer treated by radical hysterectomy and adjuvant chemoradiotherapy[J]. European Journal of Gynaecological Oncology, 2020, 41(1): 42-47.
No Suggested Reading articles found!