Please wait a minute...
European Journal of Gynaecological Oncology  2020, Vol. 41 Issue (4): 622-628    DOI: 10.31083/j.ejgo.2020.04.5436
Original Research Previous articles | Next articles
The role of sodium hydrosulfide in the proliferation and apoptosis of exogenous SB203580 pre-treated human ovarian cancer cells
Xifang Lv1, , Amanguli1, , Ping Yang1, *()
1Obstetrics and Gynecology department, The First Af filiated Hospital of the Medical College, Shihezi University, Xinjiang, Shihezi, 832008, P. R. China
Download:  PDF(405KB)  ( 201 ) Full text   ( 4 )
Export:  BibTeX | EndNote (RIS)      
Abstract  
Purpose: Explore the regulatory function of sodium hydrosulfide (NaHS) on the proliferation and apoptosis of exogenous SB203580 pre-treated human ovarian cancer SKOV3 cells. Methods: SKOV3 cells cultured in vitro were assigned to the control group, NaHS group, SB203580 group, and NaHS combined with SB203580 group. The effects and influences on cell proliferation were detected by MTT assay. Effects on the cell cycle and apoptosis were detected by flow cytometry. Western blot was used to detect the expression of P-p38MAPK. Results: Proliferation of SKOV3 cells were remarkably decreased in the NaHS, SB203580, and NaHS combined with SB203580 groups. Cell quantity in G1 phase and S phase increased and decreased remarkably, respectively. Obviously, cell apoptosis rate was increased in the combination group compared to single-drug treatment groups. P-p38MAPK expression in NaHS group was upregulated by comparing with that in control group, and that was markedly enhanced in the combination group compared to single-drug treatment group. Conclusions: NaHS inhibited proliferation and promoted apoptosis of SKOV3 cells by blocking the p38MAPK signaling pathway. Furthermore, coordination between NaHS and SB203580 regulated the proliferation and apoptosis of SKOV3 cells.
Key words:  Human ovarian cancer cells      Sodium hydrosulfide      p-P38MAPK      Cell proliferation      Cell apoptosis     
Submitted:  20 November 2019      Accepted:  31 March 2020      Published:  15 August 2020     
*Corresponding Author(s):  PING YANG     E-mail:  aabu123@163.com
About author:  Contributed equally

Cite this article: 

Xifang Lv, Amanguli, Ping Yang. The role of sodium hydrosulfide in the proliferation and apoptosis of exogenous SB203580 pre-treated human ovarian cancer cells. European Journal of Gynaecological Oncology, 2020, 41(4): 622-628.

URL: 

https://ejgo.imrpress.com/EN/10.31083/j.ejgo.2020.04.5436     OR     https://ejgo.imrpress.com/EN/Y2020/V41/I4/622

[1] F. Yan. Effects of salvianolic acid B on growth inhibition and apoptosis induction of ovarian cancer SKOV3[J]. European Journal of Gynaecological Oncology, 2016, 37(5): 653-656.
No Suggested Reading articles found!