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The importance of dysregulated miRNAs on ovarian cysts and epithelial ovarian cancer |
Ece Gumusoglu1, Tuba Gunel1, *( ), Mohammad Kazem Hosseini1, Nogayhan Seymen2, Taylan Senol3, Uğur Sezerman2, Samet Topuz4, Kılıç Aydınlı5 |
1Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Istanbul, Turkey 2Acıbadem University, School of Medicine, Department of Basic Sciences, Biostatistics, 34684, Istanbul Turkey 3Istanbul Zeynep Kamil Women and Child Diseases Training and Research Hospital, 34668, Istanbul, Turkey 4Istanbul University, Istanbul Medical Faculty, Department of Obstetrics and Gynecology, 34093, Istanbul, Turkey 5Medicus Healthcare Centre, 34365, Istanbul, Turkey |
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Abstract
Objective: Benign ovarian cysts (BOC) are the most common tumors in women of reproductive age. Usually, these cysts are harmless, but, a small number of them occasionally progress to malignancy. Among ovarian malignancies, epithelial ovarian cancer (EOC) comprises 90% and is the most important cause of gynecologic cancer-related deaths. We aimed to identify dysregulated miRNAs in patients with benign ovarian cysts compar to EOC and to healthy individuals (HI). We studied three different sample groups (serum of EOC (n = 8), HI (n = 8), and BOC (n = 8) patients) that contained the highest-quality of RNA. Microarray data were analyzed according to expression of miRNAs and target genes by bioinformatics tools. Results: When compared to EOC samples, 75 miRNAs were dysregulated in BOC samples. Sixty-six miRNAs from BOC were dysregulated when compared to HI samples. Bioinformatics analysis of BOC vs. EOC and BOC vs. HI showed that 46 miRNAs were congruent and their expression alterations were similar (up- or down-regulated). Further analysis showed that these 46 miRNAs are associated to one of three pathways involved in cancer pathogenesis. Conclusion: Several miRNAs might play a role in BOC formation and/or malignant transformation. These dysregulated miRNAs could potentially be a biomarker to distinguish between a completely BOC and one that is malignant or has potential for malignant transformation.
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Submitted: 09 June 2020
Revised: 16 October 2020
Accepted: 16 October 2020
Published: 15 February 2021
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Fund: 51472/Scientific Research Projects Coordination Unit of Istanbul University |
*Corresponding Author(s):
Tuba Gunel
E-mail: gunel@istanbul.edu.tr
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